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Bradys reagent (2,4-DNPH)

Biochemistry
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It is used primarily to test for the presence of aldehydes and ketones, as it reacts with them to form a yellow to orange precipitate.

 

Composition:
Brady's reagent contains 2,4-dinitrophenylhydrazine (DNPH) in alcohol (typically methanol or ethanol).

 

Reaction with Aldehydes/Ketones:
When the reagent is added to a sample containing an aldehyde or a ketone, it reacts with the carbonyl group (C=O) of the compound.

 

Formation of Hydrazone:
The reaction produces a yellow or orange precipitate of a hydrazone derivative, which indicates the presence of an aldehyde or ketone.